El análisis de cantos mostró divergencia acústica con una tasa de pulsos y frecuencia dominante mayor en el Clado Sur comparado con el Clado Norte y otras especies de Pseudacris. Observamos posible hibridación entre el Clado Norte con otra especie relacionada ( P. El análisis de agrupamiento genético también muestra una división genética clara que concuerda con dichos clados. brachyphona en los clados Norte y Sur basada en datos mitocondriales. Nuestra filogenia de especies apoya la hipótesis previa que separa a P. Con el uso de secuencias de ADN nuclear obtenidas a nivel genómico mediante “anchored hybrid enrichment,” datos acústicos y modelamiento ecológico, evaluamos la existencia de especies crípticas dentro de P. Sin embargo, un muestreo insuficiente y la falta de caracteres morfológicos diagnósticos and dificultado una evaluación mas completa de su estatus taxonómico. Estudios filogenéticos han sugerido la existencia de linajes divergentes dentro de P. ![]() Pseudacris brachyphona es un ejemplo de estos organismos poco estudiados debido a su carácter esquivo, distribución en parches, y periodo reproductivo corto. Aunque los Montes Apalaches han sido estudiados ampliamente, mucho de su filogeografía de anfibios continúa sin conocerse. Un factor importante para la generación de diversidad en los Montes Apalaches es la paleogeografía compleja de esta zona. This study highlights the need for genus-wide, population-level genetic assessments and integrative data sets to uncover diversity within anurans. brachyphona, by the Tennessee River and surrounding Blue Ridge Escarpment. Pseudacris collinsorum, new species, is geographically separated from its sister species, P. The new species ranges across northern Alabama, and extends into Georgia, eastern Mississippi, and southern Tennessee. In light of the genetic, acoustic, and ecological divergence between the clades, we propose to elevate the taxonomic status of the Southern clade and name this new species the Collinses' Mountain Chorus Frog ( P. Furthermore, species distribution models showed that habitat suitability for the Southern clade included pine-dominated and drier areas compared to the Northern clade. Analysis of advertisement calls shows a divergent, faster pulse rate, and higher dominant frequency call for the Southern clade in comparison to the Northern clade and other trilling chorus frogs. Admixture was observed between the Northern clade and another chorus frog species ( P. Population genetic clustering also shows a sharp genetic break, which is concordant with these clades. brachyphona into the Northern and Southern clades. ![]() Our species tree estimation supports previous mitochondrial-based phylogenetic hypotheses that separate P. Using a genome-wide nuclear data set obtained via anchored hybrid enrichment, acoustic data, and ecological modeling, we tested for the existence of cryptic species within P. brachyphona however, insufficient sampling and the lack of diagnostic morphological traits have prevented further assessment of their taxonomic status. Phylogenetic studies have suggested the existence of divergent lineages within P. The Mountain Chorus Frog ( Pseudacris brachyphona) is one example of an understudied organism due to its elusiveness, patchy distribution, and short breeding seasons. Although the Appalachian Mountains have been widely studied, much of its amphibian phylogeography remains poorly known. A major driver generating amphibian diversity in the Appalachian Mountains is the complex paleogeography of the zone.
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